22H041  邵晓奇，蔡平东，张越(广东药科大学).Infarction or reperfusion-induced cardiac autophagy: role of microRNAs（梗死或再灌注诱导的心脏自噬：microRNA的作用）

[摘要]目的 观察补阳还五汤对糖尿病大鼠视网膜炎症的保护作用及机制。方法 选取健康雄性Wistar大鼠作为研究对象，随机分为正常组与模型组。模型组大鼠采用一次性腹腔注射链脲佐菌素（STZ）建立糖尿病模型，再将造模成功的大鼠随机分为模型组、阳性对照组、补阳还五汤组。各组对应给药8周后，通过尾静脉注射荧光素标记的右旋糖酐观察大鼠视网膜血管通透性；尾静脉注射伊文思蓝染液定量检测大鼠视网膜的血管渗漏量；灌注荧光素标记的刀豆蛋白检测大鼠视网膜白细胞黏附；蛋白免疫印迹法（WB）检测大鼠视网膜组织中炎症因子TNF-α、NF-κBp65和p-NF-κBp65蛋白的表达水平。结果 与正常组相比，模型组大鼠视网膜血管渗漏和白细胞黏附量以及TNF-α和p-NF-κBp65蛋白表达水平显著升高（P<0.05）；与模型组相比，阳性对照组与补阳还五汤组中大鼠视网膜血管渗漏和白细胞黏附量,以及TNF-α和p-NF-κBp65的表达水平明显降低（P<0.05）。结论 补阳还五汤能防治由糖尿病引起的大鼠视网膜炎症反应，其作用机制可能与抑制TNF-α表达及NF-κB通路激活有关。

[关键词]糖尿病； 大鼠； 视网膜炎症； 补阳还五汤； 机制

[Abstract] Objective To observe the protective effect of Buyang Huanwu decoction (BYHWD) against retinal inflammation in diabetic rats. Methods Healthy male Wistar rats were randomly divided into normal and diabetic model groups. Diabetic rats were given a one-time intraperitoneal injection of streptozotocin (STZ) to establish a diabetic phenotype and were randomly assigned to the negative control (saline), positive control (calcium dobesilate), and BYHWD treatment groups. After 8 weeks of treatment, fluorescein-labeled dextran and Evans blue dye were injected into the tail vein to observe vascular permeability and vascular leakage in the retina, respectively. Perfusion of fluorescein-labeled concanavalin was used to detect adhesion in rat retinal leukocytes. Western blotting was used to quantify the expression levels of the inflammatory factors TNF-α, NF-κBp65, and p-NF-κBp65 in retinal tissue. Results Compared with nondiabetic rats, saline-treated diabetic rats showed significantly increased retinal vascular leakage, leukocyte adhesion, and protein levels of TNF-α and p-NF- κBp65 (P<0.05). These effects were significantly reduced in the positive control and BYHWD groups (P<0.05). Conclusion Buyang Huanwu decoction can ameliorate retinal inflammation in STZ-induced diabetic rats. Its mechanism of action may be related to the inhibition of TNF-α expression and NF-κB pathway activation.

[Key words] Diabetes; Rats; Retinitis; Buyang Huanwu decoction; Mechanism